Progress PhD Research - 2nd scope of study (2nd Year/Sem 1)
Just to share this knowledge to all of my friends. Dah mula run experiment untuk enzymatic cellulosic technology with ultrasound application. Dalam scope nie jer ada 2 jenis cellulose (substrate) yg akan apply; soluble (SCMC), lactose and insoluble (Solka-floc) with 3 different particle sizes dan 2 jenis enzyme; cellulase (Accellerase 1000) and Beta-galactosidase. Biasalah kat industry kadang2 ada yg soluble dan insoluble. Senang cerita reaction process antara cellulose dgn enzyme untuk produce glucose. Cuma state-of-the-art nya kat sini adalah capability ultrasound untuk enhance reaction rate tanpa mengubah atau menambah kuantiti substrate (Km) dan fix kuantiti enzyme. Klu kuantiti substrate bertambah, lama2 kilang pun boleh tutup....
Oklah sedikit abstract research & paper untuk publish sedikit masa lagi hehehe
High perfomance hydrolysis of Sodium Carboxymethyl Cellulose (SCMC) - Soluble Cellulose using Ultrasonication Pretreatment
Effective approaches to a practical high-performance enzymatic hydrolysis of cellulose were investigated using a ultrasonic irradiation as a pretreatment from cellulase, Accellerase 1000. Their efficacy on enhancement of reactivity was discussed based on the kinetic parameters, i.e; Michaelis constant Km, maximum reaction rate Vmax, and initial reaction rate Vi, correlating with ultrasonic conditions. A relatively ultrasonication of the cellulose clearly enhanced the enzyme reaction rate. Enhancement of Vmax without increasing Km (It was the one of the useful data for hydrolysis of cellulosic resources) by ultrasonication was effective by increasing the irradiation power and short irradiation time. The mechanisms that contribute to the enhancement were that substrate surface became rough under the influence of ultrasonic pretreatment.The roughness of surface promoted adsorption of cellulase molecules on surface of cellulose fiber. This finding is considered advantageous for the practical implementation of ultrasonic pretreatment in a cellulose hyrolysis system and cellulose saccharification process.
